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That worked, thank you.

Please add a note about this to the manual.

It's a Nikon D300. The focus system is set at single-servo autofocus and single-point AF.
I defined focus point 1, which was red, and I used it as the end focus point.

I followed the "simple focus stacking workflow" from the manual. I set the starting focus point and the end focus point as well as the focus stacking image count. Then I saw, that qdslrdashboard calculated the focus step values and set the stacking direction. Subsequently I activated the c...